Enzyme used for the mapping of hypersensitive sites in DNA – Biochemistry MCQ

The enzyme used for the mapping of hypersensitive sites in recombinant DNA research is?
A. DNA ligase
B. DNA polymerase I
C. DNase I
D. Polynucleotide kinase

Correct answer: C. DNase I (Deoxyribonuclease I)

Production of recombinant DNA

• DNA ligase is used for joining of DNA molecules.
• DNA polymerase I is used for synthesis of double stranded cDNA.
• Polynucleotide kinase is used for T-32 end labeling of DNA or RNA.

Ref: Harper’s Illustrated Biochemistry, 27th edition, p406.
Image credits: Minestrone Soup. Licensed under the Creative Commons Attribution-ShareAlike 3.0 License. https://en.wikipedia.org/wiki/File:Recombinant_formation_of_plasmids.svg