AIIMS November 2011 – MCQ 31

All of the following are required in PCR except
A. Deoxyribonucleotides
B. Thermostable enzyme
C. Dideoxyribonucleotides
D. DNA template

Correct answer : C. Dideoxyribonucleotides

A basic PCR set up requires several components and reagents. These components include:

  • DNA template that contains the DNA region (target) to be amplified.
  • Two primers that are complementary to the 3′ (three prime) ends of each of the sense and anti-sense strand of the DNA target.
  • Taq polymerase or another DNA polymerase with a temperature optimum at around 70 °C.
  • Deoxynucleoside triphosphates (dNTPs; nucleotides containing triphosphate groups), the building-blocks from which the DNA polymerase synthesizes a new DNA strand.
  • Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
  • Divalent cations, magnesium or manganese ions; generally Mg2+ is used, but Mn2+ can be utilized for PCR-mediated DNA mutagenesis, as higher Mn2+ concentration increases the error rate during DNA synthesis
  • Monovalent cation potassium ions.
Source: Wikipedia
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AIIMS November 2011 – MCQ 30

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