Biochemistry – MCQ 64 – Detection of point mutation

The following methods can be used to detect the point mutation in the beta (B)-globin gene that causes sickle cell anemia, except:
A. Polymerase chain reaction with allele-specific oligonucleotide hybridization
B. Southem blot analysis
C. DNA sequencing
D. Northem blot analysis

Correct answer : D. Northem blot analysis

Point mutation in a gene is identified by checking for defects in the DNA. Northern blot is used for analysis of RNA.

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