Evans blue in measurement of plasma volume

  • Evans blue is an azo dye with high affinity for albumin
  • It is used to measure plasma volume
  • When evans blue is administered, it tightly binds to albumin so that it does not escape into the interstitial fluid compartment – is limited to the plasma
  • By measuring the concentration of Evans blue in plasma, we can find the volume of distribution as we already know the quantity of dye administered
  • Plasma volume can also be measured using Albumin labelled with radioactive iodine

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