Method for determining the purity of protein – Biochemsitry MCQ

The most widely used method for determining the purity of a protein is?
A. Isoelectric focusing
B. Polyacrylamide gel electrophoresis
C. Ion exchange chromatography
D. High pressure chromatography

Correct answer: B. Polyacrylamide gel electrophoresis

SDS-PAGESDS-PAGE

  • The most widely used method for determining the purity of a protein is polyacrylamide gel electrophoresis (PAGE) in the presence of an anionic detergent, sodium dodecyl sulphate (SDS).
  • Electrophoresis separates the charged biomolecules based on the rates at which they migrate in an electrical field.

Ref: Harper’s Illustrated Biochemistry, 27th edition, p22-25.
Image credits: Author: Magnus Manske. Licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license. https://en.wikipedia.org/wiki/File:SDS-PAGE.jpg

Related Posts