Yield of recombinant protein synthesis – Biochemistry MCQ
Process used in an expression vector to increase yield of recombinant protein synthesis ?
A. Translation initiation
B. Promoter induction
C. Transcription terminators
D. Multiple cloning sites
Correct answer : B. Promoter induction
Expression vectors and recombinant protein synthesis
- Expression vectors are plasmids / viruses designed to regulate protein expression in a target cell
- They are used to insert a specific gene into the target cell to produce the desired protein
- They contain regulatory sequences which act as enhancer and promoter regions to bring about efficient transcription of the gene
- An inducible promoter regulates the gene expression and can increase the yield of the recombinant protein
