Yield of recombinant protein synthesis – Biochemistry MCQ

Process used in an expression vector to increase yield of recombinant protein synthesis ?

A. Translation initiation
B. Promoter induction
C. Transcription terminators
D. Multiple cloning sites

Correct answer : B. Promoter induction

Expression vectors and recombinant protein synthesis

  • Expression vectors are plasmids / viruses designed to regulate protein expression in a target cell
  • They are used to insert a specific gene into the target cell to produce the desired protein
  • They contain regulatory sequences which act as enhancer and promoter regions to bring about efficient transcription of the gene
  • An inducible promoter regulates the gene expression and can increase the yield of the recombinant protein