The best culture medium for primary isolation of H. influenzae is?
A. Chocolate agar
B. Fildes’ agar
C. Levinthal’s medium
D. Nutrient agar
Correct answer : B. Fildes’ agar
Haemophilus influenzae colonies in chocolate agar
- Fildes’ agar is made by adding peptic digest of blood to nutrient agar.
- It gives copious growth of H. influenzae and it is the best culture medium for the primary isolation of H. influenzae.
- The other media used for the isolation of H. influenzae are chocolate agar, blood agar, nutrient agar and Levinthal’s medium.
Ref: Ananthanarayan and Paniker’s Textbook of Microbiology, 9th edition, p330.
Image credits: Stefan Walkowski. Licensed under the Creative Commons Attribution-Share Alike 4.0 International license. https://commons.wikimedia.org/wiki/File:Haemophilus_influenzae.jpg