Gold standard test for measuring beta HCG – Obstetrics MCQ

Gold standard test for measuring ß HCG levels in serum is?
B. Bioassay
C. Radioimmunoassay
D. Latex agglutination test

Correct answer : C. Radioimmunoassay

Radioimmunoassay is the most sensitive test for measurement of ß HCG levels in serum.

ELISA plate ELISA plate

Immunological tests for diagnosis of pregnancy

Immunological tests for the diagnosis of pregnancy depend on the detection of ß HCG in the maternal serum or urine. They are of various types:

  • Agglutination inhibition test
  • Direct agglutination test
  • Enzyme linked immunosorbent assay (ELISA)
  • Fluoroimmunoassay (FIA)
  • Radioimmunoassay (RIA)
  • Immunoradiometric assay (IRMA)

Other uses of ß HCG testing:

  • Diagnosing ectopic pregnancy
  • Monitoring patients who have underwent in vitro fertilization and embryo transfer
  • Follow up of patients after treatment of hydatidiform mole and choriocarcinoma

Ref: DC Dutta’s Textbook of Obstetrics by Hiralal Konar, 7th ed, p66

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